Fig. 2. Nox4 is required for flagellin-induced NF-kB activation in smooth muscle cells.

a WT and Nox4 KO SMCs were stimulated with flagellin (100 ng/mL) for 1 h and stained with a p65 antibody (red) and DAPI (blue). A representative image is shown. Scale bar = 50 μm. b Nuclear-translocated p65 was quantified using the ImageJ program (N = 3, mean ± SD, **p < 0.01, ***p < 0.001). c Immunoblotting of phosphorylated IkBα and IkBα