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. 2019 Jul 10;51(7):78. doi: 10.1038/s12276-019-0275-6

Fig. 5. Deficiency of Nox4 decreased RhoA and Rac activation in response to flagellin.

Fig. 5

a RhoA activity assay. WT and Nox4 KO SMCs were stimulated with flagellin (100 ng/mL) for 60 min and subjected to a pulldown assay for RhoA activity. The amount of immunoprecipitated GTP-RhoA was analyzed by immunoblot analysis with anti-RhoA. Total cell lysates were analyzed for RhoA expression as a loading control. b Rac activity assay. WT and Nox4 SMCs were stimulated with flagellin (100 ng/mL) for 60 min. Cell lysates were incubated with bead-bound GST-PAK-RBD for 1 h. The bound proteins on the beads were subjected to immunoblot analysis with anti-Rac. Total cell lysates were analyzed for Rac expression as a loading control. c Immunoblot analysis of phosphorylated JNK in cells stimulated with flagellin (100 ng/mL) for the indicated times. d Effect of a JNK inhibitor (SP600125) on JNK phosphorylation in WT SMCs