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. 2019 Sep 10;11(3):24. doi: 10.1038/s41368-019-0055-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — IF staining of the NK-1 receptor and the changes in GFAP in TGs and SGC cultures. a, b IF staining of the NK-1 receptor in rat TG slices and SGC cultures. (green: NK-1; red: GS; blue: DAPI). c, d IF staining of GFAP on rat TG slices and SGC cultures. (red: GFAP; blue: DAPI). Scale bars: 50 μm. The representative WB images and the bar graph shown in (e) illustrated that the expression of GFAP in the CFA-treated groups was higher than that in the vehicle-treated groups. The representative WB images and the bar graph shown in (f) illustrate that the expression of GFAP in SP-treated SGCs was higher than that in vehicle-treated SGCs. (n = 6, *P < 0.05 compared with the vehicle group)