Figure 7.
Birthdate analyses of GABAergic neurons ectopically produced from the RL. A, Scheme for the birthdate analyses by BrdU incorporation. Pregnant mice (E10.5 or E14.5) were given intraperitoneal injections of BrdU, and the embryos (Tg–Atoh1–Cre;R26R and Atoh1Ptf1a/+;Tg–Atoh1–Cre;R26R) were fixed at E18.5, followed by immunostaining. B–I, Triple immunolabeling with β-gal (green), BrdU (red), and cell type-specific markers (blue), such as calbindin (B–E) and Pax2 (F–I). B′–I′, High-magnification views of rectangular regions in B–I, respectively. Small panels below B′–I′ are single colored or merged pictures corresponding to the rectangular regions in B′–I′. Yellow arrows and arrowheads indicate colocalization of BrdU and the marker. White arrows and arrowheads indicate triple colocalization of β-gal, BrdU, and calbindin or Pax2. All are sagittal sections. Top is dorsal, and left is rostral. Scale bars: B–I′, 50 μm; small panels below B′–I′, 10 μm.