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. 2019 Jul 19;51(7):82. doi: 10.1038/s12276-019-0282-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematics of the three CD episomal vectors are shown. b RT-PCR was conducted to confirm the expression of exogenes in each CD vector in 293T cells. The pCXLE-GW plasmid is an empty vector that was used as a negative control. c The number of generated colonies was counted. This experiment was performed in triplicate. d Quantitative PCR was conducted to evaluate the expression levels of OCT4 and NANOG in CD-iPSCs, control-iPSCs, and ESCs (H9 and HUES9). e Representative images of CD-iPSCs treated with or without cytosine