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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Nov 12;143(5):1865–1877.e4. doi: 10.1016/j.jaci.2018.08.053

FIG 3. Genetic deficiency and overexpression of CRF2 induces divergent effects on stimuli induced MC degranulation. A:

FIG 3.

β-hex % release from IgE-sensitized WT and CRF2−/− BMMCs stimulated with DNP (32 ng/mL). B, C: β-hex release from WT and CRF2−/− BMMCs stimulated with A23187 (B) and c48/80 (C). D: Transmission electron microscopy analysis of WT and CRF2−/− BMMCs stimulated with IgE/DNP. E,F: β-hex release from RBL-2H3 MCs transfected with CRF2-overexpressing plasmid or eGFP control or CRF2-siRNA. Data are means ± SEM from a representative experiment with n=3 (bone marrow donors) per group. Experiments were repeated in n=6 (A-C) or 2 (E-F) independent replicates. *Significance between groups was determined by an unpaired two-tailed t-test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001