FIG 6. CRF2−/− BMMCs exhibit heightened intracellular Ca²⁺ store release and expression of SOCE channels.

BMMCs derived from WT and CRF₂^−/− mice were loaded with Fluo 4, and intracellular Ca²⁺ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca²⁺ traces for experiments conducted under Ca²⁺-replete (A) or Ca²⁺-free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), TRPC1 (E, H), and Orai (F, I) in WT and CRF₂^−/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p<0.05, **p<0.01.