Figure 7.
CRTH2 deficiency and the CRTH2 antagonists reverse tumor (colon 26)-induced decreases in social behavior. CRTH2+/+ and CRTH2−/− mice were subcutaneously injected with 1 × 106 colon 26 cells or cell-free medium. A–E, Tumor diameter (A), locomotor activity (B), food intake (C), duration engaged in object exploratory behavior (D), and social interaction (E) were determined at the indicated days (E, at day 7) after the inoculation. F–H, At day 7, 30 min after CRTH2+/+ mice were intraperitoneally injected with vehicle (Veh), 30 mg/kg ramatroban (Ram; F and G) or 30 mg/kg CAY10471 (CAY; H), the duration engaged in object exploratory behavior (F) and social interaction (G, H) was analyzed. Data are expressed as the mean ± SEM (A–E, n = 4–8; F, G, n = 4–5; H, n = 8–9). *p < 0.05, **p < 0.01 versus control, ##p < 0.01 versus CRTH2+/+ mice by three-way (A–D) or two-way (E–H) ANOVA followed by Tukey—Kramer test.