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. 2014 Feb 12;34(7):2544–2558. doi: 10.1523/JNEUROSCI.3500-13.2014

Figure 7.

Figure 7.

Leucokinin transmission by 5-HT1B-positive ABLK neurons is involved in the regulation of rearing. A–B″, Double staining for mCD8::GFP expressed by 5-HT1B-Gal4 (green) and leucokinin (purple). A, Frontal view of the brain. A′, Enlarged view of A. B, B′, Expression in the VNC. 5-HT1B-Gal4 expression in A1 and A2 is weaker compared with that in other neuromeres. Signals for 5-HT1B-Gal4 and leucokinin overlap in the ABLK neurons (B–B″), but not in the brain (A, A′). The white arrowheads indicate the midline. C, Inhibition of leucokinin neurons in c127-Gal4,UAS-GFP/+;;UAS-Shits/+ larvae increased rearing (n = 10–25). D, shRNA-mediated knockdown of leucokinin in 5-HT1B-Gal4 neurons increased rearing (n = 8–12). Behavioral assays in C and D were performed at 32°C. *p < 0.05; **p < 0.01 (Tukey–Kramer post hoc test).