Video 8. Cholinergic activation does not trigger large changes in cytosolic Ca⁺⁺ in DD spines in the absence of ATR.

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DOI: 10.7554/eLife.47918.021

Three examples of GCaMP6s changes recorded after activation of cholinergic neurons every 2.5 s (pink circle) in control worms grown in the absence of ATR. Scale bar = 2 µm. Pseudo-colored with Rainbow Dark LUT. Arrows denote spines. Asterisk (left panel) marks DD neuron soma.