. Author manuscript; available in PMC: 2020 Oct 1.

Published in final edited form as: FEBS J. 2019 Jun 21;286(20):4036–4059. doi: 10.1111/febs.14954

Table 3.

Catalytic performance of RhlA from P. aeruginosa upon substitution of non-conserved residues on the cap-domain (relative to RhlA from B. glumae).

Mutation	Relative activity (%)^ab	Mutation	Relative activity (%)^ab
A144D	78 (± 2)^d	S173Y	10 (± 1)^c
L145H	60 (± 4)^d	H177Y	0
E147A	85 (± 5)^d	A179T	73 (± 4)^d
L148A	106 (± 1)^d	A182P	39 (± 2)
D149G	86 (± 11)^d	T183R	0
K151N	8 (± 2)^c	G184D	84 (± 3)^d
S152L	29 (± 2)^c	Y186Q	0
A153H	24 (± 7)^c	E187D	0
H156Q	66 (± 9)^d	R190A	0
E160D	80 (± 6)^d	D194N	63 (± 3)^d
K164R	156 (± 13)^d	A198E	105 (± 3)^d
P168R	0	L199M	4 (± 2)^c
R169I	16 (± 2)^c	R202E	0

Catalytic activity is relative to the WT RhlA_Pa performance with standard deviation (SD) obtained from triplicate experiments.

Rhamnolipid congeners detected by LC/MS.

Low production yields of this mutant hampered proper identification and quantification of congeners.

No differences were observed in the RL congener pattern with respect to WT.