. Author manuscript; available in PMC: 2020 Oct 1.

Published in final edited form as: FEBS J. 2019 Jun 21;286(20):4036–4059. doi: 10.1111/febs.14954

Table 4.

Rhamnolipid congener pattern observed upon expression of the RhlA_Pa-A182P/RhlB_Pa variant with respect to RhlA_Pa-WT/RhlB_Pa in a BGR1ΔrhlA background.

Variant	C₈ (%)^*	C₁₀ (%)^*	C₁₂ (%)^*
RhlA_Pa-WT	5.4 (± 0.6)	74.3 (± 0.6)	20.3 (± 0.6)
RhlA_Pa-A182P	7.9 (± 0.6)	65.1 (± 1.1)	27.0 (± 1.3)

Fatty acid contribution were calculated from LC/MS negative mode analysis of congeners C₈-C₈ (m/z 301.3), Rha-C₈-C₈ (m/z 447.3), Rha-Rha-C₈-C₈ (m/z 593.5), C₈-C₁₀/C₁₀-C₈ (m/z 329.3), Rha-C₈-C₁₀/Rha-C₁₀-C₈ (m/z 475.4), Rha-Rha-C₈-C₁₀/Rha-Rha-C₁₀-C₈ (m/z 621.6), C₁₀-C₁₀ (m/z 357.3), Rha-C₁₀-C₁₀ (m/z 503.4), Rha-Rha-C₁₀-C₁₀ (m/z 649.5), C₁₀-C₁₂/C₁₂-C₁₀ (m/z 385.3), Rha-C₁₀-C₁₂/Rha-C₁₂-C₁₀ (m/z 531.4), Rha-Rha-C₁₀-C₁₂/Rha-Rha-C₁₂-C₁₀ (m/z 677.5), C₁₂-C₁₂ (m/z 413.6), Rha-C₁₂-C₁₂ (m/z 559.7), and Rha-Rha-C₁₂-C₁₂ (m/z 705.5). No others congeners longer than C₁₂ were detected.