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Bacteroides fragilis down-regulate FPN protein independently of hepcidin. (A) Control or BCM-treated BMDMs were processed for isolation of membrane fractions and then probed for FPN via western blotting. Sodium-potassium ATPase was used for normalization. The western blot is representative of three independent experiments. (B) BMDMs exposed to BCM overnight were analyzed for mRNA expression levels of hepcidin by qRT-PCR. Results are shown as mean ± S.D. from 5 independent replicates.
