Fig. 7.

Exposure to BCM can overcome effects of iron chelation on Salmonella-induced cytokine secretion by macrophages. BMDMs were pre-treated with BCM or BCM plus deferoxamine overnight, followed by infection with S. Typhimurium in the presence of the corresponding pre-treatment reagent. Supernatants were collected 7 hrs after infection and ELISAs were carried out to measure IL-6 (A) or IL-1β (B), and the cells were lysed to quantitate intracellular bacterial burden (C). Results are shown as mean ± S.D. from 3 technical replicates of one experiment representative of three independent experiments. One way ANOVA test was performed on ranks. *p < 0.05.