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. Author manuscript; available in PMC: 2020 Oct 28.
Published in final edited form as: Angew Chem Int Ed Engl. 2019 Sep 9;58(44):15904–15909. doi: 10.1002/anie.201908713

Figure 4.

Figure 4.

A selected cyclic peptide ligand CycH8a and its binding and inhibition of HDAC8. (A) The structure of 5FAM-CycH8a. The sequence of CycH8a, highlighted in blue and red, was selected from phage display; (B) Fluorescence polarization analysis of 5FAM-CycH8a binding to HDAC8. Data for a linear counterpart of 5FAM-CycH8a is also included; (C) A diagram to show a fluorogenic HDAC8 activity assay scheme; (D) The IC50 determination of 5FAM-CycH8a inhibition of HDAC8 using the assay shown in C.