Skip to main content
. Author manuscript; available in PMC: 2020 Oct 22.
Published in final edited form as: Circulation. 2019 Aug 29;140(17):1409–1425. doi: 10.1161/CIRCULATIONAHA.119.040629

Figure 1. Bmpr2 haploinsuffiriency renders rats susceptible to PAH following 5-LO-mediated pulmonary inflammation.

Figure 1.

(A and B) Assessments of right ventricular systolic pressure (RVSP, A) and right ventricular hypertrophy ((weight ratio of right ventricle (RV) to left ventricle (LV) plus septum (S), RV/LV+S, B)) of Bmpr2+/Δ527bp rats at the 3, 6, 9 or 12 months of age. Hemodynamics measurements of WT cohort in the same age groups were analyzed in parallel. (C–G) Assessments of RVSP (C), RV/LV+S (D) and percentage of survival (G) of WT or Bmpr2+/394527bp rats administered with intratracheal instillation of adenovirus overexpressing Alox5 (gene encoding for 5-LO, AdAlox5) or GFP-tagged control vector (AdGFP). Rats not subjected to virus treatments were used as controls. Hemodynamics measurements were performed at post-operative week 3. Female and male rats were randomly assigned to different experimental groups and compared (E and F). (H and I) Representative western blots and quantification of BMPR2 in lung tissue collected from WT and Bmpr2+/Δ527bp rats assigned in different treatment groups at post-operative week 3. Actin was used as loading control. n=3. (J and K) Representative hematoxylin and eosin (H&E) and Elastin stain images and quantification of percentage vascular muscularization. n=6; scale bar, 100μm. For panels A–D, data are presented in box-and-whiskers plots showing minimal to maximal values and all data points; for panels E and F, data are presented in violin plots showing all data points; for panel K, data are presented as mean and SEM; for panel G, data are presented by Kaplan-Meier survival plot; for panel I, data are presented as mean and SEM; Mann-Whitney test for panels A, B, E, F and K; Kruskal-Wallis test for panels C, D and I; ns, not significant, *p< 0.05, **p < 0.01, ***p<0.001.