Figure 6.
Changes in mitochondrial membrane potential (MMP) and mRNA expression of mitofusin 1 and 2 (MFN1 and MFN2) by SWCNTs and IAV. A TMRE method was used to determine the MMP in SAEC exposed to different doses of SWCNTs (A), IAV (B), and exposed to different doses of SWCNTs for 24 hours following IAV (MOI=0.5) infection for 24 hours (C). The mRNA expression of MFN1 (D) and MFN2 (E) were measured by qPCR in SAEC which were exposed to SWCNTs at different doses for 24 hours followed by IAV infection at MOI=0.5 for another 24 hours. A total of six samples per group were measured and data presented as Mean ± SD. Asterisks “*” above each bar indicate statistically significant differences compared to control. (P<0.05)