FIG. 4.

Free energy barrier extracted by committor inversion, G_CI(q), using the observed committor (solid lines) and the analytic approximation of Eq. (11) (dashed lines and darker shade of color). We varied the height of the hidden molecular barrier ($\mathrm{\Delta }{G}_o^{‡}=3$, 8.1, and 16 k_BT, from top to bottom) and the linker stiffness (κ_l = 1.5, 2.5, and 5 k_BT/[q]², green, red, and blue lines, respectively). We used the matched-harmonic free energy function from Eq. (12) with Δx^‡ = 1.5 [x]. For reference, each panel shows the respective hidden molecular profile G_o (gray solid line).