Figure 1.

RT-PCR analysis of 8 selected genes differentially expressed in NP69, CNE1, CNE2, S26 and S18 cells through microarray analysis. (A) Hierarchical clustering was performed based on mRNA profiling in 4 paired primary NPC tissues compared by corresponding benign adjacent tissues from the nasopharyngeal carcinoma patients. The results of hierarchical clustering revealed that distinct mRNA expression profiles between the samples; (B) quantitative RT-PCR analysis of 8 genes in NPC cells lines compared with NP69 cell lines. The graph depicts the mean of these fold-changes ± SD of three independent amplifications normalized to GAPDH gene expression. RT-PCR, real time-polymerase chain reaction; NPC, nasopharyngeal carcinoma.