Table 5. Discrepant investigation for samples with discordant results.

Patient	FA ME detection	CSF culture	Target PCR assay	Additional analysis or supplemental testing	Clinical diagnosis from medical records	Final resolution of FA ME result
1	Negativea	E. coli	E. coli	Blood culture = E. coli	Bacterial meningitis	False negative
2	Negative	E. coli	Negative	Blood culture = E. coli	Bacterial meningitis	True negative
3	E. coli K1	Negative	E. coli	Blood culture = E. coli	Bacterial meningitis	True positive
4	E. coli K1	Negative	E. coli	Blood culture = negative	Bacterial meningitis	True positive
5	E. coli K1	Negative	Negative	Blood culture = S. agalactiae	Septicemia	False positive
6	E. coli K1	Negative	E. coli	Blood culture = E. coli	Bacterial meningitis	True positive
7	E. coli K1	Negative	E. coli	Blood culture = E. coli	Bacterial meningitis	True positive
8	S. agalactiae	Negative	Negative	Blood culture = negative	Bacterial meningitis	False positive
9	S. agalactiae	Negative	S. agalactiae	Blood culture = negative	Bacterial meningitis	True positive
10	S. agalactiae	Negative	S. agalactiae	Blood culture = S. haemolyticus	Bacterial meningitis	True positive
11	S. pneumoniae	Negative	Negative	S. pneumoniae antibody = positive	Bacterial meningitis	False positive
12	S. pneumoniae	Negative	S. pneumoniae	S. pneumoniae antibody = positive	Bacterial meningitis	True positive
13	S. pneumoniae	Negative	S. pneumoniae	Blood culture = negative	Bacterial meningitis	True positive
14	S. pneumoniae	Negative	S. pneumoniae	Blood culture = negative	Bacterial meningitis	True positive
15	Negative	S. pneumoniae	Negative	S. pneumoniae antibody = positive	Bacterial meningitis	True negative
16	L. monocytogenes	Negative	L. monocytoge	Blood culture = L. monocytoge	CNS infectious	True positive
17	Negative	H. influenzae	Negative	Blood culture = negative	Bacterial meningitis	True negative
18	N. meningitidis	Negative	N. meningitidis	Blood culture = negative	Bacterial meningitis	True positive
19	Cr. neoformans/C. gattii	Negative	Cr. Neoformans/C.gattii	Blood culture = negative	Cryptococcal meningoencephalitis	True positive

^a, for E. coli, only the K1 capsular type is detected by the FilmArray ME panel.