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. 2019 Oct 15;93(21):e00930-19. doi: 10.1128/JVI.00930-19

FIG 3.

FIG 3

CD8+ T cell activity and neutralizing antibody responses do not contribute to reduced FrMLV infection in bumble mice. (A) FrMLV-infected cells in the pLNs (n = 4) of B6 and bumble (bmb) mice treated with isotype or CD8+ T cell-depleting antibodies (s.c., 5 dpi). (B to D) Percentage of CD8+ T cells that are CD107a+ (surface staining) after stimulation with FrMLV-specific peptide for 17 h (B) or granzyme A+ (C) or granzyme B+ (D) in pLNs (n = 5) of FrMLV-infected B6 and (bmb) mice (s.c., 6 dpi). Cells from pLNs from uninfected (UI) B6 and bmb mice are shown as controls. ns, P > 0.05. (E) FrMLV-neutralizing activity in serially diluted sera from B6 and bmb mice (n = 7 to 9) at indicated time points after FrMLV infection. (F) Neutralizing titers (IC50) in sera from the experiment shown in panel E. ns, P > 0.05. Statistical comparisons were performed using nonparametric Mann-Whitney tests (two-tailed).