FIG 6.

Downregulation of p62 still occurs during infection with the Δγ₁34.5 virus but in a proteasome-independent manner. (A) HEL cells were uninfected or infected with the Δγ₁34.5 virus (3 PFU/cell), and whole-cell lysates were collected at 3, 6, 9, and 30 h after infection. Equal amounts of protein were analyzed by immunoblot analysis using an anti-p62 antibody. β-Actin served as a loading control. (B) HEL cells were uninfected or infected with the Δγ₁34.5 virus (5 PFU/cell) and were either untreated or treated with MG132 (10 μM) at 1 h postinfection. Whole-cell lysates were collected at 3, 9, and 24 h after infection. Equal amounts of protein were analyzed by immunoblot analysis using an anti-p62 antibody and an anti-ICP0 antibody. β-Actin served as a loading control. All experiments were repeated two independent times, and representative Western blots are depicted.