FIG 1.

Intranuclear flow cytometry analysis of ASP expression in multiple cell lines. Chronically infected myeloid (U1 and OM-10.1) and lymphoid (J1.1, ACH2, 8E5, H9/IIIB, H9/CC, H9/MN, and H9/RF) cell lines and their uninfected parental myeloid (U937 and HL-60) and lymphoid (Jurkat, A3.01, and H9) cell lines were fixed and permeabilized with a FoxP3 staining kit to allow detection of nuclear proteins. Then, the cells were stained with the anti-ASP 324.6 (light-blue curve and line) or a control mouse IgG (open curve with red line) labeled with Alexa Fluor 647. Finally, the cells were washed, fixed, and analyzed by flow cytometry. All the chronically infected cell lines analyzed showed nuclear expression of ASP to varying degrees. The results shown are representative of several independent experiments. The mean fluorescence intensity (MFI) of samples stained with the 324.6 MAb is reported in each panel.