FIG 10.
ASP and HIV-1 gp120 colocalize on the surfaces of primary human monocyte-derived macrophages infected with HIV-1Ada-M. Macrophages were generated from freshly isolated PBMC and infected as described in Materials and Methods. At day 15 postinfection, the cells were surface stained with Alexa Fluor 488-labeled anti-gp120 (green) and Alexa Fluor 647-labeled anti-ASP 324.6 (red). In addition, the cells were stained with DAPI to identify the nuclei. After washing, the cells were fixed and analyzed by confocal microscopy. The overlay of the images taken in the green (HIV-1 gp120), red (ASP), and blue (DAPI) channels shows the presence of both HIV-1 gp120 and ASP on the cell surface. In addition, ASP and HIV-1 gp120 present a significant degree of colocalization. Bar, 5 μm for all panels. The results shown are representative of three independent experiments.