FIG 7.

Flow cytometry analysis of ASP expression on the surface in multiple cell lines. Chronically infected myeloid (U1C8 and OM-10.1) and lymphoid (J1.1, ACH2, 8E5, H9/IIIB, H9/CC, H9/MN, and H9/RF) cell lines and their uninfected parental myeloid (U937 and HL-60) and lymphoid (Jurkat, A3.01, and H9) cell lines were treated with PMA as indicated in Materials and Methods to reactivate HIV-1 expression. Then, the cells were surface stained with anti-ASP 324.6 (light-blue curve and line) or a control mouse IgG (open curve with red line) labeled with Alexa Fluor 647. After washing, the cells were fixed and analyzed by flow cytometry. All the chronically infected cell lines analyzed showed cell surface expression of ASP to varying degrees. The results shown are representative of several independent experiments.