FIG 9.
ASP and HIV-1 gp120 colocalize on the surfaces of primary human CD4+ T cells infected with HIV-1Ada-M. CD4+ T cells were isolated, activated, and infected as described in Materials and Methods. At day 7 postinfection, the cells were surface stained with Alexa Fluor 488-labeled anti-gp120 (green) and Alexa Fluor 647-labeled anti-ASP 324.6 (red). After washing, the cells were fixed and analyzed by confocal microscopy. The overlay of the images taken in the green (HIV-1 gp120) and red (ASP) channels shows the presence of both HIV-1 gp120 and ASP on the cell surface. In addition, ASP and HIV-1 gp120 present a significant degree of colocalization. Bar, 5 μm for all panels. The results shown are representative of three independent experiments.