FIG 2.

Lnc-MxA has profound effects on IAV replication. (A to C) 293T cells were seeded into 12 wells and transfected with the indicated amounts of lnc-MxA plasmid or empty vector (EV) for 28 h. (A) The level of lnc-MxA was determined by RT-qPCR. (B and C) The cells were then infected with influenza virus A/WSN/33 at an MOI of 0.5 for 12 h. The cell lysates were harvested for immunoblotting with antibodies against the indicated proteins (B), and the supernatants were collected for a plaque assay (C). (D to F) 293T cells were transfected with control (sh-luc) or lnc-MxA shRNA plasmids. (D) The efficiency of shRNA-based knockdown of lnc-MxA was determined by RT-qPCR. (E and F) The cells were then infected with influenza virus A/WSN/33 at an MOI of 1 for 12 h. The cell lysates were harvested for immunoblotting with antibodies against the indicated proteins (E), and the supernatants were collected for a plaque assay (F). n = 3; means and SD; **, P < 0.01.