Lnc-MxA negatively regulates antiviral immune responses. (A and B) Lnc-MxA-overexpressing A549 cells, knockdown cells, or control cells were stimulated with poly(I·C) (0.5 μg/ml) for 6 h. The supernatants were harvested and used to treat fresh A549 cells for 6 h. Then, total RNA was prepared, and the ISGs were examined by RT-qPCR (n = 3; means and SD; *, P < 0.05; **, P < 0.01). (C to F) Lnc-MxA-overexpressing A549 cells, knockdown cells, or control cells were stimulated with poly(I·C) (0.5 μg/ml) for 6 h. The supernatants were harvested and used to treat fresh A549 cells for 6 h. Then, the A549 cells were infected with GFP-tagged VSV for 14 h. The percentage and MFI of VSV-GFP-positive A549 cells were determined by flow cytometry (n = 3; means and SD; *, P < 0.05; **, P < 0.01).