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Lnc-MxA affects IAV replication in an IFN-β-dependent manner. WT and IFNAR1 knockout (IFNAR1 KO) 293T cells were transfected with lnc-MxA or empty-vector plasmid for 28 h. The cells were then infected with influenza virus A/WSN/33 at an MOI of 0.01 for 24 h. The cell lysates were harvested for immunoblotting with antibodies against the indicated proteins (A), and the supernatants were collected for a plaque assay (B) (n = 3; means and SD; **, P < 0.01; N.S, not significant).
