FIG 5.

Replication of HSV-1(F) and accumulation of viral proteins in HEp-2 cells exposed to IFN-γ. (A) Replicate cultures of HEp-2 cells were mock treated or exposed to 250 ng/ml of IFN-γ for 24 h before infection or concurrently with 1 PFU of HSV-1(F) per cell. After 1 h, the inoculum was replaced with fresh medium. (B and C). The HEp-2 cells were mock treated or exposed to IFN-γ 24 h before infection or concurrently with 5 PFU of HSV-1(F) per cell. The cells were harvested at 7 or 18 h after infection, solubilized, subjected to electrophoresis in denaturing gels, and probed with specific antibodies to ICP0, ICP4, ICP8, ICP27, VP16, and US11. GAPDH served as a loading control.