FIG 4.
Confirmation of binding specificity for antibodies produced against TSWV-interacting protein (TIP) (peptides) by dot blot analysis. Peptide antigens were diluted to 100 μg/ml (for cyclophilin, enolase, endoCP-GN, endoCP-V, and mATPase), and 2.5 mg/ml (for CP-V), and 2 μl of each peptide was used for each test. PBS buffer and preimmune serum (500,000 × dilution) were used as controls. All six diluted peptides and two controls were loaded onto six nitrocellulose membrane strips. Each strip was initially incubated with the homologous primary antibody (0.5 μg/ml, generated in mice), followed by incubation with anti-mouse-HRP (1:5,000 dilution). Each membrane strip was developed independently.