TABLE 1.
Identification of Frankliniella occidentalis larval proteins bound to purified virions of tomato spotted wilt virus in 2D gel overlays
| Spot no. | Fo seq contig matcha | Mascot score (P < 0.05) | % coverageb | No. of matched peptides | Peptide sequence(s) | BLASTX annotation (E < 10−20)c | Conserved motif(s) (E < 10−10) |
|---|---|---|---|---|---|---|---|
| 1 | contig01248 | 279 | 37 | 4 | R.AQQPYQQYLQNQQFQNYQQR.A, R.AAAAPILQYSNDVNPDGSFQYSYQTGDGISAQAAGFT.R, K.DAEAQVVQGSYSYTGPDGVVYTVNYIADENGYR.A, K.ALPYYNQQQATYQQQQAAYQR.P | Endocuticle structural glycoprotein | Chitin_bind_4 |
| CL4854Contig1 | 363 | 29 | 7 | R.VFFDMTVDGQPAGR.I, R.ALCTGEQGFGYK.G, R.VIPNFMCQGGDFTNHNGTGGK.S, R.KFADENFQLK.H, K.HTGPGIMSMANAGPNTNGSQFFITTVK.T, K.TSWLDNKHVVFGSVIEGMDVVK.K, K.HVVFGSVIEGMDVVK.K | Cyclophilin | Cyclophilin_ABH | |
| 2 | contig01248 | 77 | 10 | 1 | K.DAEAQVVQGSYSYTGPDGVVYTVNYIADENGYR.A | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 3 | contig00018 | 196 | 25 | 4 | R.FGGALGGYNLAQTSQYHIQTDEGPER.Y, R.LEDGTVVGTYGWVDADGYLR.L, R.PYYPSSTPAVSLVSSTPR.P, R.PYYPTSTPAVVSSTPR.P | Uncharacterized, similar to cuticular protein | Chitin_bind_4 |
| contig14634 | 134 | 22 | 3 | R.GYISELPGTYDANSNSVIPEYDGIAVTHNGFR.Y, K.AGSFGYVDPFGIR.R, R.VIYYNTSPGSGFQVR.K | Uncharacterized, similar to cuticular protein | none identified | |
| CL4900Contig1 | 113 | 10 | 3 | R.GYISELPGTYDASSNSVIPEYDGIAVTHNGFR.Y, K.AGSFGYVDPFGIR.R, R.VIYYNTSPGSGFQVR.K | Uncharacterized, similar to cuticular protein | Chitin_bind_4 | |
| 4 | CL1591Contig1 | 76 | 5 | 1 | K.QESVYTAAQPAISTYK.K | Flexible cuticular protein | Chitin_bind_4 |
| 5 | CL1591Contig1 | 89 | 5 | 1 | K.QESVYTAAQPAISTYK.K | Flexible cuticular protein | Chitin_bind_4 |
| 6 | CL4854Contig1 | 615 | 30 | 9 | R.VFFDMTVDGQPAGR.I, R.ALCTGEQGFGYK.G, R.VIPNFMCQGGDFTNHNGTGGK.S, R.KFADENFQLK.H, K.FADENFQLK.H, K.FADENFQLKHTGPGIMSMANAGPNTNGSQFFITTVK.T, K.HTGPGIMSMANAGPNTNGSQFFITTVK.T, K.HVVFGSVIEGMDVVK.K, K.KVVVADCGQLS | Cyclophilin | Cyclophilin_ABH |
| 7 | CL4706Contig1 | 554 | 47 | 17 | R.GNPTVEVDLVTELGLFR.A, R.AAVPSGASTGVHEALELR.D, K.AIDNVNNIIAPELIK.S, K.EIDELMLK.L, K.LGANAILGVSLAVCK.A, K.HIADLAGNTNIILPTPAFNVINGGSHAGNK.L, K.LAMQEFMILPTGASSFK.E, K.FGLDSTAVGDEGGFAPNILNNK.E, K.EGLTLIIDAIAK.A, K.VEIGMDVAASEFYK.D, K.VEIGMDVAASEFYKDGQYDLDFK.N, K.DGQYDLDFKNPNSDK.S, K.LTDLYMEFIK.E, K.EFPMVSIEDPFDQDHWDAWTTITGK.T, K.TNIQIVGDDLTVTNPK.R, K.VNQIGSVTESIQAHLLAK.K, R.SGETEDTFIADLVVGLSTGQIK.T | Enolase | Metal_binding |
| contig12136 | 96 | 24 | 2 | R.QGDVVQGSYSLVEPDGSR.R, R.TVEYTADPVNGFNAVVHK.D | Cuticular protein | Chitin_bind_4 | |
| contig14594 | 102 | 3 | 1 | R.TVDYTADPVNGFNAVVR.K | Nuclear cap-binding protein | RRM_NCPB2d, Chitin_bind_4 |
|
| CL504Contig1 | 113 | 19 | 2 | K.AAVAVDTDYDPNPSYNYAYDIHDSLTGDAK.S, R.TVEYTADPVNGFNAVVHK.E | Cuticular protein | Chitin_bind_4 | |
| 8 | CL4706Contig1 | 407 | 43 | 15 | R.GNPTVEVDLVTELGLFR.A, R.AAVPSGASTGVHEALELR.D, K.AIDNVNNIIAPELIK.S, K.HIADLAGNTNIILPTPAFNVINGGSHAGNK.L, K.LAMQEFMILPTGASSFK.E, K.FGLDSTAVGDEGGFAPNILNNK.E, K.EGLTLIIDAIAK.A, K.VEIGMDVAASEFYK.D, K.VEIGMDVAASEFYKDGQYDLDFK.N, K.DGQYDLDFKNPNSDK.S, K.LTDLYMEFIK.E, K.EFPMVSIEDPFDQDHWDAWTTITGK.T, K.TNIQIVGDDLTVTNPK.R, K.VNQIGSVTESIQAHLLAK.K, R.SGETEDTFIADLVVGLSTGQIK.T | Enolase | Metal_binding |
| CL504Contig1 | 99 | 12 | 1 | K.AAVAVDTDYDPNPSYNYAYDIHDSLTGDAK.S | Cuticular protein | Chitin_bind_4 |
a
De novo-assembled contigs from F. occidentalis transcriptome derived by Roche 454/Sanger EST library hybrid (18).
b
Highest percent coverage obtained among the three picking gels used to collect protein spots for identification using ESI-mass spectrometry.
c
NCBI BLASTX search of the nonredundant protein database with the matching Fo nucleotide sequence contig query.
d
Chimeric contig (= ambiguous annotation); domains occur in opposite orientation on different ORFs.