TABLE 2.
Identification of Frankliniella occidentalis larval proteins bound to recombinant GN in 2D gel overlays
| Spot no. | Fo seq contig matcha | Mascot score (P < 0.05) | % coverageb | No. of matched peptides | Peptide sequence(s) | BLASTX annotation (E < 10−20)c | Conserved motif (E < 10−10) |
|---|---|---|---|---|---|---|---|
| 1 | CL4310Contig1 | 595 | 27 | 12 | R.AAELSSILEER.I, K.NIQADEMVEFSSGLK.G, K.GMALNLEPDNVGIVVFGNDK.L, K.GMALNLEPDNVGIVVFGNDKLIK.E, R.TGAIVDVPVGDDLLGR.V, K.TALAIDTIINQQR.F, K.YTIIVAATASDAAPLQYLAPYSGCAMGEYFR.D, K.HALIIYDDLSK.Q, R.EAYPGDVFYLHSR.L, R.EVAAFAQFGSDLDAATQQLLNR.G, K.QGQYVPMAIEEQVAVIYCGVR.G, K.IVTDFLASFNAASK | Mitochondrial ATP synthase α-subunit | AtpA |
| 2 | CL4310Contig1 | 302 | 12 | 5 | K.GMALNLEPDNVGIVVFGNDK.L, R.TGAIVDVPVGDDLLGR.V, R.VVDALGDAIDGK.G, K.HALIIYDDLSK.Q, K.IVTDFLASFNAASK | Mitochondrial ATP synthase α-subunit | AtpA |
| 3 | CL4382Contig1 | 633 | 43 | 9 | R.SSVVSQSVPVVSK.T, K.SVPQYQQQYQTVSQYQSVPQYQQQVVVK.S, K.SVPQYQQQVVVK.S, K.SAPVYSQVHHVVEQQAAPVLLR.H, R.TAFVPQYDSVSVSASAQPK.Y, K.ILSQVQEFDPAGIYR.V, R.VNFQTENGIQSAETGSVK.D, R.ASGAHLPQVPEEIQR.S, R.SLELNAAQPQKYDQDGNLVSQF | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 4 | CL4382Contig1 | 137 | 15 | 3 | R.SSVVSQSVPVVSK.T, K.SVPQYQQQYQTVSQYQSVPQYQQQVVVK.S, R.VNFQTENGIQSAETGSVK.D | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 5 | CL4382Contig1 | 169 | 22 | 6 | R.SSVVSQSVPVVSK.T, K.SVPQYQQQVVVK.S, R.VNFQTENGIQSAETGSVK.D, R.ASGAHLPQVPEEIQR.S, R.AAAEHGVAIVCPDTSPR.G, K.ACQAVNMPVVLQMR.E | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 6 | CL4382Contig1 | 1,154 | 53 | 11 | R.SSVVSQSVPVVSK.T, K.SVPQYQQQYQTVSQYQSVPQYQQQVVVK.S, K.SVPQYQQQVVVK.S, K.SAPVYSQVHHVVEQQAAPVLLR.H, R.HVEQEIPAYQSVQHVHQPVYQSVQHVAAHHVAAPVVSR.T, R.TAFVPQYDSVSVSASAQPK.Y, K.ILSQVQEFDPAGIYR.V, R.VNFQTENGIQSAETGSVK.D, R.VNFQTENGIQSAETGSVKDIQAK.D, R.ASGAHLPQVPEEIQR.S, R.SLELNAAQPQKYDQDGNLVSQF | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 7 | CL4382Contig1 | 413 | 74 | 8 | R.SSVVSQSVPVVSK.T, K.SVPQYQQQYQTVSQYQSVPQYQQQVVVK.S, K.SVPQYQQQVVVK.S, R.TAFVPQYDSVSVSASAQPK.Y, R.VNFQTENGIQSAETGSVK.D, R.VNFQTENGIQSAETGSVKDIQAK.D, R.ASGAHLPQVPEEIQR.S, R.SLELNAAQPQKYDQDGNLVSQF | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 8 | CL4382Contig1 | 355 | 29 | 7 | R.SSVVSQSVPVVSK.T, K.SVPQYQQQYQTVSQYQSVPQYQQQVVVK.S, K.SVPQYQQQVVVK.S, R.VNFQTENGIQSAETGSVK.D, R.VNFQTENGIQSAETGSVKDIQAK.D, R.ASGAHLPQVPEEIQR.S, R.SLELNAAQPQKYDQDGNLVSQF | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 9 | CL4382Contig1 | 155 | 5 | 2 | R.VNFQTENGIQSAETGSVK.D, R.VNFQTENGIQSAETGSVKDIQAK.D | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 10 | CL4382Contig1 | 130 | 4 | 1 | R.VNFQTENGIQSAETGSVK.D | Endocuticle structural glycoprotein | Chitin_bind_4 |
| 11 | CL4382Contig1 | 150 | 13 | 7 | R.SSVVSQSVPVVSK.T, K.SVPQYQQQYQTVSQYQSVPQYQQQVVVK.S, K.SVPQYQQQVVVK.S, R.VNFQTENGIQSAETGSVK.D, R.TAFVPQYDSVSVSASAQPK.Y, R.VNFQTENGIQSAETGSVK.D, R.VNFQTENGIQSAETGSVKDIQAK.D, R.ASGAHLPQVPEEIQR.S | Endocuticle structural glycoprotein | Chitin_bind_4 |
a
De novo-assembled contigs from F. occidentalis transcriptome derived by Roche 454/Sanger EST library hybrid (18).
b
Highest percent coverage obtained among the three picking gels used to collect protein spots for identification using ESI-mass spectrometry.
c
NCBI BLASTX search of the nonredundant protein database with the matching Fo nucleotide sequence contig query.