FIG 3.

Effects of IP6 on WT MA trimerization and CT binding. (A) WT MA proteins, incubated in either the absence (−phytic) or the presence (+phytic) of IP6 (phytic acid), were UV cross-linked and detected as monomers (1), dimers (2), and trimers (3), as described in the legend to Fig. 2A. The far-right panel shows the percentages of trimers relative to monomers averaged from two independent experiments, with standard deviations as indicated. The P value for the observed difference is 0.0307. (B) Immunoblots of bound and total unbound MA proteins from incubations with GST-CT or GST beads in the absence (−) or presence (+) of IP6. At the far right, relative percentages of bound versus total MA protein levels were derived from either two (GST) or six (GST-CT) independent experiments. The P value for the observed differences in GST-CT binding is 0.0003.