FIG 7.

Presentation to, and priming of, CD8⁺ T cells by OVA₂₅₇ expressed in different loci of rMVA and without a functional TK. (A) MVA genome maps showing HindIII fragments and the site of insertion of OVA antigenic constructs and the promoter (arrow). The “m” denotes the minigene (OVA₂₅₇). (B to D, left) Extent of cell surface presentation of MHC-I:OVA₂₅₇ complexes on DC2.4 cells infected with viruses. (B to D, middle) Mice were infected with rMVA viruses expressing versions of OVA₂₅₇ (as shown) and, 7 days later, the responses to peptides were measured. (B to D, right) Using the data shown in the middle graphs, the OVA₂₅₇-specific response was normalized by dividing by the total VACV-specific response. Means and standard errors of data from at least nine mice combined from three independent experiments are shown (*, P < 0.05).