FIG 4.

IFI44L expression decreases antiviral responses. (A) HAP-1 cells transfected with the NT siRNA were transfected with poly(I·C) or treated with 2,000 U/ml of IFN-α. IFI44L mRNA levels were measured by RT-qPCR. (B) HAP-1 cells transfected with NT siRNA or with two IFI44L-specific siRNAs were transfected with poly(I·C) or treated with 2,000 U/ml of IFN-α. IFN-β (top panels), IFIT2 (middle panels), and IFN-λ1 (bottom panels) mRNA levels were measured by RT-qPCR and are represented as fold change values compared to values in mock-treated cells. In panels A and B, bars represent SDs of results determined using duplicate wells. Three different experiments were performed with similar results. P values determined by using a Student's t test are indicated. (C) Cells left mock treated, transfected with poly(I·C), or treated with 2,000 U/ml of IFN-α were infected with rVSV-GFP (MOI, 0.1). Virus production was analyzed at 24 hpi. Bars represent SDs of results determined using duplicate wells. P values determined by using a Student's t test are indicated. Three different experiments were performed, with similar results.