FIG 7.

Binding of IFI44L to FKBP5 does not disrupt FKBP5 interaction with IKKβ or IKKε. Human 293T cells were transiently cotransfected with plasmids expressing IFI44L-HA, FKBP5-FLAG, and either myc-IKKβ (A) or His-IKKε (B). Then, Co-IPs using a resin bound to FLAG antibody (to immunoprecipitate FKBP5) (B) were performed, and the presence of IFI44L (anti-HA tag antibody), FKBP5 (anti-FLAG tag antibody), IKKβ (anti-myc tag antibody), and IKKε (anti-His tag antibody) was analyzed by Western blotting in the initial cellular extracts (input; left) and after the Co-IP [CoIP anti-FLAG (FKBP5); right]. Molecular weight markers (in kilodaltons) are indicated on the right. Three different experiments were performed, with same results.