FIG 11.

HCMV virion proteins colocalize with the Rab7 marker of endosomes in NT2 cells. NT2 cells grown on coverslips were incubated with HCMV (AD169; MOI = 1) for 2 h at 37°C. The cells were then fixed and processed for indirect immunofluorescence for the indicated virion protein (red) and Rab7 (green). Nuclei were stained with Hoechst (blue). Boxed areas are shown in magnification (zoom). White arrowheads indicate colocalization. Representative images of three independent biological replicates are shown.