FIG 6.

CPMV and eCPMV stimulation induces immune cell profile changes in the tumor microenvironment. (A) Peritoneal cavity wash cells were collected from tumor-bearing mice on day 35 after tumor inoculation. The cells were incubated with CPMV or eCPMV (or PBS as a control) for 24 h and then analyzed by flow cytometry. (B) CPMV and eCPMV/p were i.p. injected into the peritoneal cavity of tumor-bearing mice on day 35 after tumor inoculation. The peritoneal cavity wash cells were collected 24 h later and analyzed by flow cytometry. Data are means ± SEM (n = 2 for the eCPMV/i ex vivo stimulation data set; n = 3 for all other data sets). Statistical significance was calculated by one-way ANOVA with a Tukey test. *, P < 0.05; **, P < 0.01; ***, P < 0.0005; ****, P < 0.0001.