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. 2019 Oct 15;93(21):e00943-19. doi: 10.1128/JVI.00943-19

FIG 2.

FIG 2

Crb3 and CLDN15 KO enhance transduction with hepatotropic AAV serotypes. (A to C) Flow cytometric analysis after transduction of Scr, CLDN15 KO, and Crb3 KO with self-complementary vectors AAV9 at 50,000 viral genomes/cell (A), AAV8 at 20,000 viral genomes/cell (B), and AAV3 at 10,000 viral genomes/cell (C). (D) Flow cytometric analysis of GFP expression after transfection of pTR-CBh-scGFP in Scr, CLDN15, and Crb3 KO cell lines. (E to G) Transduction of Scr, CLDN15 KO, and Crb3 KO Huh7 with various doses of AAV1 (E), AAV2 (F), and AAV9 (G) packaging CBA-luciferase. (H) Flow cytometric analysis of transduction of Scr, CLDN15 KO, and Crb3 KO lines with rAd5-CMV-GFP. A two-tailed unpaired t test was used (*, P < 0.05; **, P < 0.01; ***, P < 0.005).