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Characterization of clonal Crb3 CRISPR KO cell line. (A) High-throughput sequencing of Crb3 indel site in Scr and Crb3 KO cells. (B) Quantification of the percent mutant reads for Crb3 in the Scr and clonal CRISPR Crb3 KO cell lines. (C to E) Immunofluorescent staining of Scr and Crb3 KO Huh7 cells with DAPI (blue) and E-cadherin (C), occludin (D), and ZO-1 (E).
