FIG 6.

Crb3 KO augments cell surface binding of galactose-dependent AAV capsids. (A) Binding curve for AAV9 on Scr and Crb3 KO cells. (B) Binding of AAV2i8g9 on Scr and RNF121 KO cells. (C and D) Structural model of the AAV9 trimer (C) with a closeup of its galactose footprint, highlighted in yellow (D). (E and F) Structural model of the AAV2i8g9 trimer (E), along with a closeup of its engrafted galactose footprint highlighted in yellow (F). The AAV8 derived residues are highlighted in red. A two-tailed unpaired t test was used (*, P < 0.05; **, P < 0.01; ***, P < 0.005).