TABLE 1.
Target | Oligonucleotide sequence (5′–3′) |
|
---|---|---|
Forward | Reverse | |
Cldn15 gRNA | caccgCGAGGTGGCGACGGGCATCA | aaacTGATGCCCGTCGCCACCTCGc |
Crb3 gRNA | caccgCACCTACCGGCCCAGTAGCG | aaacCGCTACTGGGCCGGTAGGTGc |
Crb3 AmpliconEZ | ATGCCATTCAGGTGGAGGTG | CCATCAGAGGCTGGTGTCTC |
Crb3 qRT-PCR | CCTTCATCCACCAGCTCCAG | GCCAAGAGGGAGAAGACCAC |
Luciferase qPCR | AAAAGCACTCTGATTGACAAATAC | CCTTCGCTTCAAAAAATGGAAC |
Laminin qPCR | GTTAACAGTCAGGCGCATGGGCC | CCATCAGGGTCACCTCTGGTTCC |
ITR qPCR | AACATGCTACGCAGAGAGGGAGTGG | CATGAGACAAGGAACCCCTAGTGATGGAG |