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. 2019 Oct 15;93(21):e00943-19. doi: 10.1128/JVI.00943-19

TABLE 1.

Oligonucleotides used in cloning and qPCR

Target Oligonucleotide sequence (5′–3′)
Forward Reverse
Cldn15 gRNA caccgCGAGGTGGCGACGGGCATCA aaacTGATGCCCGTCGCCACCTCGc
Crb3 gRNA caccgCACCTACCGGCCCAGTAGCG aaacCGCTACTGGGCCGGTAGGTGc
Crb3 AmpliconEZ ATGCCATTCAGGTGGAGGTG CCATCAGAGGCTGGTGTCTC
Crb3 qRT-PCR CCTTCATCCACCAGCTCCAG GCCAAGAGGGAGAAGACCAC
Luciferase qPCR AAAAGCACTCTGATTGACAAATAC CCTTCGCTTCAAAAAATGGAAC
Laminin qPCR GTTAACAGTCAGGCGCATGGGCC CCATCAGGGTCACCTCTGGTTCC
ITR qPCR AACATGCTACGCAGAGAGGGAGTGG CATGAGACAAGGAACCCCTAGTGATGGAG