FIG 8.

Comparison of ORV variant infections in different C. elegans wild isolates. N2, JU1580, MY10, and JU2572 C. elegans wild isolates were infected with the ORV variants JUv2572 and JUv1580. For each C. elegans isolate, five L4 larvae were infected with 50 μl of the ORV viral filtrate (either JUv1580 or JUv2572), and cultures were propagated for two/three generations at 20°C. Infections were performed in triplicates. ORV infections were monitored on F2 adult hermaphrodites by FISH using a Biosearch probe (Cal Fluor red 610) targeting the ORV RNA2 molecule. (A) Proportion of infected F2/F3 adult hermaphrodites. The total number of animals scored are given below the histograms. Bars represent the standard deviations among replicates. P < 0.001 (***) and P < 0.05 (*) in a glm taking the viral variant knowing the host genotype into account, with each virus effect being compared to JUv1580 on the host C. elegans JU1580. (B) Number of infected cells per infected F2/F3 adult hermaphrodite. The total number of infected animals are given below the histograms. (C) At the adult stage, the intestine of Caenorhabditis elegans is composed of 9 rings of intestinal cells (2 or 4), ring 1 being close to the pharynx and ring 9 to the rectum. (D and E) Distribution of infected cells along C. elegans (Cel) intestine considering all the C. elegans (D) isolates or each of them separately (E).