FIG 7.

Characterization of other conserved genes in P. aeruginosa. (A) Relative expression profiling patterns of PA0285, PA0290, PA4367, and PA5017 in P. aeruginosa meta-array analyses. In total, 162 conditions were tested for expression analyses of all four genes, and we found that there was no significant difference within these conditions. The x axis represents 162 different conditions, and the y axis indicates the expression values calculated from a meta-analysis of the GEO data sets. The expression value is shown on a log₂ scale. (B) Relative biofilm analysis of four DGC gene mutants. The biomasses were detected for P. aeruginosa PAO1 and DGC mutants; CV staining was used and three independent experiments were performed to calculate the final biomass formation. The results are presented as the biofilm formation relative to wild-type PAO1. (C) Relative Psl production of four DGC gene mutants. The diameters of swimming bacteria were measured, and relative swarming was calculated by evaluating the wild-type PAO1. The standard errors are shown.