TABLE 2.
Primers used in this study
| Strain or purpose | Primer | Sequence (5′→3′)a |
|---|---|---|
| A12 ΔpcfV | A12-pcfV F-1 | TTGGCCTCGATATGCTTCGAGGG |
| A12-pcfV R-2GA-Km | GCCATTTATTATTTCCTTCCTCTTTTAGCAACTCCCCTTAATTAGATGATCTACCG | |
| A12-pcfV F-3GA-Km | ATATTTTACTGGATGAATTGTTTTAGTAGAGGTGCGTAATCACTTCTCATCATGTC | |
| A12-pcfV R-4 | CCCTGCAACTGTGGAAGG | |
| A12 ΔpcfO | A12-pcfO F-1 | GCTGGCCGATCTGGCG |
| A12-pcfO R-2GA-Km | GCCATTTATTATTTCCTTCCTCTTTTACTTGTAAACGTACCATGATCTTCTCAC | |
| A12-pcfO F-3GA-Km | ATATTTTACTGGATGAATTGTTTTAGTAGAGGTAAATTAAGATACTAAGCCCG | |
| A12-pcfO R-4 | CCTTACCCTGTCTGGTGGAG | |
| A12 ΔpcfFEG | A12-pcfFEG F-1 | GTAAGATTAGAGTTGAATCCCCTC |
| A12-pcfFEG R-2GA-Km | GCCATTTATTATTTCCTTCCTCTTTTAATTTTGTTCCTCCTTTTTCTTCTG | |
| A12-pcfFEG F-3GA-Km | ATATTTTACTGGATGAATTGTTTTAGTAGATAGCTCTAAATGTATTTGTAAATGG | |
| A12-pcfFEG R-4 | GGCAGAAGAATCACCAATCCC | |
| A12 ΔpcfRK | A12-pcfRK F-1 | GGTGGGGTGGAAAGTCAATCAG |
| A12-pcfRK R-2GA-Km | GCCATTTATTATTTCCTTCCTCTTTTAGAAATACTATTTTGAGCG | |
| A12-pcfRK F-3GA-Km | ATATTTTACTGGATGAATTGTTTTAGTAGACAACAATTACTTTTT | |
| A12-pcfRK R-4 | GTCAAGGTCAAAGGACGGGGC | |
| A12 Δpeg.1179 | A12-peg.1179 F-1 | GGTGCTGACGAACAAATGC |
| A12-peg.1179 R-2GA-Km | GCCATTTATTATTTCCTTCCTCTTTTAGCTGGACATAAAAATCACCTCACG | |
| A12-peg.1179 F-3GA-Km | ATATTTTACTGGATGAATTGTTTTAGTAGAGGGGAGTTGCATGTTTTCAATCC | |
| A12-peg.1179 R-4 | GGGGGCTATGCTGCACC | |
| qRT-PCR | A12 pcfK 1116 F | CTAGAGCCACACTAC |
| A12 pcfK 1116 R | GCCTCTAGCATTGGCG | |
| A12 pcfR 1117 F | CGTAGCAAAGTGGATTGTCCGATTATC | |
| A12 pcfR 1117 R | CTCTTTGTTCTCTTCTCAGATGAGC | |
| A12 pcfFEG 1118 F | GTTGACATGGAAGCAAGTGCTGG | |
| A12 pcfFEG 1118 R | CAATTAACCAAGCCATAGTCCC | |
| A12 pcfFEG 1119 F | GCCGGCAACTTTCGC | |
| A12 pcfFEG 1119 R | CTCCTAGCATATGCAAAATAGC | |
| A12 pcfFEG 1120 F | CCGACAAACGGATTAGACCC | |
| A12 pcfFEG 1120 R | CTCCTTCATGTATAATTCCTATATGTTC |
a
Underlining indicates insertion of Km sequences.