FIG 3.

Fluorescence and ROS detection of cells after infection by S. eriocheiris. (A) The relative fluorescence activity was determined at 24 and 48 h postinfection with S. eriocheiris. (B) Apoptotic cells were observed clearly under fluorescence microscope. Scale bar, 100 μm. (C) Effects of S. eriocheiris on S2 cell ROS generation. FCM detects DCF fluorescence to indicate the production of ROS in the S2 cells. Statistical significance is indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001). The data are means ± the SD for three independent experiments.