FIG 4.

Dynamics of S. eriocheiris entry into S2 cells. (A) S. eriocheiris was labeled with the green fluorescent dye Alexa 488 (green). CLSM images of the experimental group at 48 h are shown. Scale bar, 10 μm. (B) Absolute real-time PCR was conducted to detect the copy number of S. eriocheiris in S2 cells. The data are means ± the SD for three independent experiments. (C) TEM images of S2 cells infected with S. eriocheiris for 48 h and control cells. Magnifications: left, ×700 (scale bar, 5 μm); right, ×1,200 (scale bar, 2 μm). High-magnification images of inclusion bodies and major intracellular vacuolization are shown. The arrow indicates the location of the inclusion body where the S. eriocheiris gathers. N, nucleus; S, Spiroplasma inclusion bodies; C, vacuolization.