FIG 6.

IL-6 mediates JAK/STAT pathways that contribute in the expression of proinflammatory and phagolysosome-related genes at early infection. (A) RAW 264.7 cells were treated with IL-6 siRNA prior to B. abortus infection and total proteins were isolated at 4 and 24 h p.i. The expression and phosphorylation of JAK1, JAK2, STAT1, and STAT3 proteins were evaluated by Western blotting assay. (B) RAW 264.7 cells were treated with different siRNAs prior to Brucella infection. The total RNA was isolated, and the transcriptional profiles of IL-6-regulated genes were assessed by qRT-PCR at 4 h p.i. The data represent means ± the SD of triplicate experiments. The asterisk indicates a significant difference (*, P < 0.05).