FIG 3.

C. burnetii replication and viability are normal in GNPTAB^−/− HeLa cells. (A) The growth of C. burnetii in GNPTAB^−/− cells is similar to that in parental cells. Genome equivalents were determined using qPCR of dotA. (B) The viability of C. burnetii grown in GNPTAB^−/− cells is normal. C. burnetii from HeLa cells at 3 dpi was used to infect monolayers of Vero cells, and CCVs were enumerated 5 dpi by a fluorescent infectious focus-forming unit (FFU) assay. Graphs represent the means ± SD from 3 independent experiments.